Journal: iScience

Article Title: Targeted deletion of Interleukin-3 results in asthma exacerbations

doi: 10.1016/j.isci.2022.104440

Figure Lengend Snippet: Analysis of T and B cells in total lung cells of OVA-treated IL-3 −/− mice (A andB) Flow cytometry analysis of CD19 + CD45R + B cells (n = 5) (a) and CD103 + cDCs (n = 4/4/5/5) (b) cells in total lung cells isolated from BALB/c WT and IL3 −/− mice with and without asthma. (C) ELISA analysis of the IL-4 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15). (D and E) CD4 + and CD8 + T cells (d) and Foxp3 + CD25 + CD4 + T reg (e) (n = 10 (CD4 + cells), n = 5 (CD8 + , T reg )). A representative dot plot is shown for each group. Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Article Snippet: Spleen CD4 + CD62L + T cells were positively sorted by magnetic bead isolation using the mouse CD4 + CD62L + T cell isolation kit (MACS, Miltenyi Biotec, Bergisch-Gladbach, Germany) in accordance to the manufacturer’s protocol.

Techniques: Flow Cytometry, Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: iScience

Article Title: Targeted deletion of Interleukin-3 results in asthma exacerbations

doi: 10.1016/j.isci.2022.104440

Figure Lengend Snippet: Increased T H 2 cytokines in the lung of IL-3 −/− asthmatic mice (A) Flow cytometry analysis of GATA3 + CD4 + T H 2 cells and in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 5). A representative dotplot is shown for each group. (B–D) ELISA analysis of the IL-5, GM-CSF, and IL-13 level in cell culture supernatants obtained from total lung cells of mice with and without asthma (n = 14/14/15/15 (IL-5 and IL-13); n = 13/14/14/14 (GM-CSF)). Data are presented as means ± SEMs. two-way ANOVA was used to calculate statistical significance. ∗p ≤ 0.05; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

Article Snippet: Spleen CD4 + CD62L + T cells were positively sorted by magnetic bead isolation using the mouse CD4 + CD62L + T cell isolation kit (MACS, Miltenyi Biotec, Bergisch-Gladbach, Germany) in accordance to the manufacturer’s protocol.

Techniques: Flow Cytometry, Cell Culture, Enzyme-linked Immunosorbent Assay